A Frictionless End-To-End Service
Countagen provides LockSeq as an end-to-end service model from DNA-In to Report-Out. LockSeq is designed eliminate the operational and infrastructure barriers of high-plex gene-editing validation.
LockSeq Service Offering
Countagen provides LockSeq as an end-to-end service model designed to eliminate the operational and infrastructure barriers of high-plex gene-editing validation. Focus on your therapeutic discovery while we deliver the sequence-level truth.
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Standardized workflow
Scalable and flexible panel design
Automated data analysis
User-friendly reporting
Your Validation Partner
By operating as a specialized service provider, Countagen enables quick and accurate on- and off-target confirmation at scale without capital expenditure.
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Project Scoping
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Panel Customization
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Technical Execution
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Consensus Reporting
We support your project design by working together on the ideal characterization parameters for your needs.
We design padlock probes for your specific on- and off-target coordinates (up to 1,000 sites).
LockSeq library preparation and sequencing are performed using our ligation-locked circularization chemistry.
You receive a comprehensive, UMI-corrected report with absolute variant allele quantification in 15 days.
Enabling High-Fidelity and Massive Scalability
Sequence-Level Truth & Resolution
Detect rare variants with absolute confidence. Our ligation-locked chemistry circularizes the target molecule before amplification, preserving the original genomic signal. By achieving high sensitivity with a suppressed noise level by consensus sequencing, we provide the high-resolution evidence required across development stages
High-Integrity Target Recovery
Ensure your characterization is complete. LockSeq is engineered to minimize target dropout by breaking the complexity wall of traditional multiplex assays. By reducing potential molecular interactions, we provide uniform and consistent coverage across every targeted locus, eliminating data gaps.
Scalable Panel Flexibility
Scale and pivot your research without workflow friction. Our modular "mix-and-match" architecture allows you to add and combine target coordinates without hassle. LockSeq chemistry produces inherently neutral circular templates compatible with all sequencing platforms, integrating seamlessly into your existing NGS infrastructure.
Join Our Pilot Phase
We are currently onboarding a select group of academic and industrial partners for structured technical pilots. Countagen functions as your specialized analytical layer: you provide the genomic coordinates and DNA samples; we deliver high-fidelity, UMI-corrected NGS reports.
Demonstrate the high-fidelity performance of the LockSeq platform on your panels and samples. Achieve the sequence-level truth required for actionable decision making by removing data blind spots.