Your Off-Target Confirmation Partner — From Coordinates to Report
A Frictionless End-To-End Service
You define the targets. We handle the rest. LockSeq runs as a complete service — probe design, library prep, sequencing, analysis, and reporting — delivered in 15 business days. No instrument investment. No bioinformatics overhead.
What's Included in Every LockSeq Study
Countagen provides LockSeq as an end-to-end service model designed to eliminate the operational and infrastructure barriers of high-plex gene-editing validation. Focus on your therapeutic discovery while we deliver the sequence-level truth.
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We review your edit design, gRNA sequences, and nominated off-target coordinates. Where needed, we can supplement with in silico nomination. Turnaround: Usually within 24 hours from first contact to project specification.
We design padlock probes for all your on- and off-target loci — up to 1,000+ sites per reaction. Probes are validated in silico before synthesis. Mix-and-match of new targets across development stages without panel redesign.
LockSeq library preparation and sequencing are performed in our Stockholm laboratory. Illumina and Oxford Nanopore platforms confirmed.
You receive a UMI-corrected variant allele frequency report with indel profiling, coverage uniformity charts, and raw data (FASTQ + BAM). Standard turnaround: 15 business days from DNA receipt.
Transparent Starting Points
LockSeq service pricing scales with panel size and sample number.
Request a quote for your specific panel configuration. Most projects are scoped within 24 hours.
Why Choose LockSeq
Sequence-Level Truth & Resolution
LockSeq uses gap-fill ligation to lock onto each target molecule before amplification. UMI-tagged reads are collapsed into consensus calls that suppress sequencing noise. Result: 0.1% VAF sensitivity with minimal false positives — even across 1,000-plex panels.
Fewer Dropouts. Better Coverage.
Ensure your characterization is complete. LockSeq is engineered to minimize target dropout by breaking the complexity wall of traditional multiplex assays. By reducing potential molecular interactions, we provide uniform and consistent coverage across every targeted locus, eliminating data gaps.
1 to 1,000+ Sites. Same Workflow.
Add, remove, or reconfigure target coordinates without redesigning the entire assay. LockSeq panels scale from single-site on-target confirmation to above 1,000-plex off-target characterization in one reaction. Sequencer-agnostic — Illumina and ONT confirmed.