Comprehensive On- and Off-Target CRISPR Validation

LockSeq utilizes a ligation-based molecular lock to deliver high-resolution sensitivity and specificity. Achieve minimal target dropout and uniform coverage for your most challenging R&D panel.

VALIDATION GAP

Overcoming the Limitations of Multiplex PCR

Validating gene editing activity at scale remains a technical hurdle. Current PCR-based amplicon sequencing (like rhAmpSeq) often forces an unacceptable trade-off: high sensitivity is obscured by non-specific amplification, bias, and significant target dropout in high-plex panels.

LockSeq replaces the inherent bias of PCR with a PCR-free molecular lock. By utilizing Gap-Fill Padlock Probes, we ensure that high-fidelity, target-specific molecules are circularized and prepared for sequencing, delivering the resolution required for rigorous preclinical analysis.

LockSeq Workflow
PLATFORM ADVANTAGES

Overcoming the Limitations of Multiplex PCR

High Specificity & Sensitivity

Our enzymatic capture chemistry establishes a "molecular lock" on every target, fundamentally reducing the false-positive background noise typical of multiplexed PCR. This provides the clean signals necessary for high-confidence lead optimization.

Minimal Target Dropout

Designed for scalability without data loss. While competitive assays often suffer from coverage gaps in high-plex runs, LockSeq is engineered for uniformity, delivering high assay success rates even on complex 100+ site panels.

Platform Agnostic Precision

LockSeq libraries are optimized for high performance across major sequencing chemistries. Achieve consistent results and high VAF concordance whether utilizing Illumina for depth or Oxford Nanopore for rapid turnaround.

Karolinska Institute
EARLY ACCESS

Join Our Pilot Phase

We are currently onboarding a limited number of academic and industrial partners for technical validation pilots. We function as your specialized back-end service: you send DNA; we deliver UMI-corrected NGS reports.

To demonstrate LockSeq's performance on your most challenging panels while providing you with high-resolution validation data without the need for capital equipment or in-house optimization.

EARLY ACCESS PROGRAM