Precision Target Capture Through A Molecular Lock
LockSeq shifts the analytical paradigm from stochastic amplification to deterministic capture. Our patented circularizable probes "lock" onto the target sequence, decoupling identification from bulk amplification. This ensures that the sequence information of the original genomic fragment is preserved as a single molecule from the very first step of the workflow.
The LockSeq Workflow
Gap-fill molecular locks circularize only upon precise base pairing, enabling sequence-specific capture of predefined loci (Hardenbol et al. 2005). Single-probe targeting prior to amplification, preserves quantitative accuracy and minimizes bias and dropout.
UMI-Native Consensus Calling
Every genomic fragment is tagged with a Unique Molecular Identifier (UMI) at the point of capture by a LockSeq probe—before any amplification. This enables consensus-based error correction (Hiatt et al. 2013). UMI-defined read groups suppress stochastic sequencing errors and improves confidence in low-frequency off-target detection.
LockSeq Enables High-Fidelity NGS-Based On- and Off-Target Confirmation
Accuracy
Enables quick and accurate on- and off-target confirmation at scale
Data Integrity
Minimizes dropouts and delivers uniform target coverage
Panel Flexibility
Supports a standardized workflow with panel flexibility across development stages